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Lipid Peroxidation Assay (MDA) Kit (C/F)

Posted on May 6, 2021 by Jordan

Example type:

Urine, plasma, cell culture extracts, tissue extracts

Product overview

The Lipid Peroxidation Assay (MDA) (Colorimetric/Fluorometric) Kit (ab118970) provides a convenient tool for the sensitive detection of Malondialdehyde (MDA).

In the lipid peroxidation assay protocol, MDA in the sample reacts with thiobarbituric acid (TBA) to generate an MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

Lipid peroxidation assay protocol summary:

– add TBA solution to samples and standards, incubate at 95ÂșC for 60 min, cool in an ice bath for 10 min
– transfer to microplate wells
– analyze with a microplate reader
For increased sensitivity, precipitate with n-butanol, centrifuge, dry, and resuspend the pellet before analysis.

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