Urine, plasma, cell culture extracts, tissue extracts
The Lipid Peroxidation Assay (MDA) (Colorimetric/Fluorometric) Kit (ab118970) provides a convenient tool for the sensitive detection of Malondialdehyde (MDA).
In the lipid peroxidation assay protocol, MDA in the sample reacts with thiobarbituric acid (TBA) to generate an MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
Lipid peroxidation assay protocol summary:
– add TBA solution to samples and standards, incubate at 95ºC for 60 min, cool in an ice bath for 10 min
– transfer to microplate wells
– analyze with a microplate reader
For increased sensitivity, precipitate with n-butanol, centrifuge, dry, and resuspend the pellet before analysis.