MITOchondrial DYNamics variation portal
The ACO2 gene homepage – Variation OPA1 mutation database
Example type: Urine, plasma, cell culture extracts, tissue extracts Product overview The Lipid Peroxidation Assay (MDA) (Colorimetric/Fluorometric) Kit (ab118970) provides a convenient tool for the sensitive detection of Malondialdehyde (MDA). In the lipid peroxidation assay protocol, MDA in the sample reacts with thiobarbituric acid (TBA) to generate an MDA-TBA adduct. The MDA-TBA adduct can be… Continue reading
Focus on developing and manufacturing ELISA test kits for your academic research Elabscience offers thousands of enzyme-linked immunosorbent assay (ELISA) kits for researchers. Species include humans, mice, rats, rabbits, monkeys, pigs, etc. As a professional ELISA kit manufacturer and supplier, our ELISA test kits have been of strict quality. -Controlled to ensure the accuracy of… Continue reading
The Viagen DirectPCR DNA Extraction System is a single tube system for the rapid preparation of DNA from mouse tails, ear muffs, yolk sac, and culture cells. Patent-pending components developed by scientists at Viagen Biotech Inc. allow the resulting DNA extracts to be compatible with genomic PCR for genotyping. Crude biological sample extracts are not… Continue reading
The UKBiobank is a prospective cohort study including approximately 500,000 UK residents (ages 40-69 years) recruited from 2006-2010 in whom extensive genetic and phenotypic investigations have been and continue to be done. All analyses involve the use of genetic : Phenotypic data from consenting UKBiobank participants. To benchmark performance of AutoMitoC, Array-based mtDNA-CN estimates were… Continue reading
Mitochondrial sequences Detailed sample and variant quality control are described in the supplementary methods. In special consideration of mtDNA-CN as the GWAS phenotype, we also removed variants within “NUMTs”, which refer to regions of the nuclear genome that exhibit homology to the mitochondrial genome Due to past transposition of mitochondrial sequences. After quality control, 359,689… Continue reading
To interrogate mtDNA-CN as a potential determinant of human diseases, we performed extensive genetic investigations in up to 395,781 participants from the UKBiobank study. We first developed and validated a novel method for biobank-scale mtDNA-CN investigations that leverages SNP array intensities, called “AutoMitoC”. Various analyses were then conducted to build on previous publications regarding the… Continue reading
While previous studies demonstrate that mtDNA-CN is a biomarker of mitochondrial activity associated with various diseases, evidence suggests that it may also play a direct and causative role in human health and disease. Forle, in cases of mtDNA depletion syndrome, wherein rare defects in nuclear genes responsible for replicating and/or maintainingNA lead to deficient mtDNA-CN.… Continue reading
Mitochondria are semi-autonomous organelles present in nearly every human cell that execute fundamental cellular processes including oxidative phosphorylation, calcium storage, and apoptotic signalling. Mitochondrial dysfunction has been implicated as the underlying cause for many human disorders based on mechanistic in vitro and in vivo studies . Complementary evidence comes from recent epidemiological studies that measure mitochondrial DNA Copy Number… Continue reading